Insertion junction: LMJ.RY0402.243894_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.243894
Chromosome:chromosome 16
Location:6155046
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g675500 FKB16-5,FKB8,FKB16E Peptidyl-prolyl cis-trans isomerase, FKBP-type; (1 of 25) PTHR10516 - PEPTIDYL-PROLYL CIS-TRANS ISOMERASE 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACCGCGTTACCTGGTCGTACGGTGCCA
Internal bar code:TAATGAAATCTAACGTTAAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:279
LEAP-Seq percent confirming:98.0732
LEAP-Seq n confirming:509
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGAGTCGTTCTTATCAGC
Suggested primer 2:TTCTCCCTGCTCCTACTCCA