| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.243894 |
| Chromosome: | chromosome_16 |
| Location: | 6155047 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g675500 | FKB16-5,FKB8 | Peptidyl-prolyl cis-trans isomerase, FKBP-type | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCTGATTCCGTATTGCAGTAATGCGCGGC |
| Internal bar code: | GACTCCGTCATATCGCTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1214 |
| LEAP-Seq percent confirming: | 98.9579 |
| LEAP-Seq n confirming: | 2279 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGAGTCGTTCTTATCAGC |
| Suggested primer 2: | TTCTCCCTGCTCCTACTCCA |