| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.038466_1 | Cre04.g219000 | (1 of 2) PTHR10887//PTHR10887:SF333//PTHR13779//PTHR13779:SF20 - DNA2/NAM7 HELICASE FAMILY // SUBFAMILY NOT NAMED // HOLLIDAY JUNCTION DNA HELICASE RUVB-RELATED // SUBFAMILY NOT NAMED | CDS | 73 | |
| LMJ.RY0402.038466_2 | Cre10.g449750 | PKS1 | (1 of 1) 1.1.1.100//1.3.1.39//2.3.1.38//2.3.1.39//2.3.1.41//2.3.1.85//3.1.2.14//4.2.1.59 - 3-oxoacyl-[acyl-carrier-protein] reductase // Enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) / Enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) // [Acyl-carrier-protein] S-acetyltransferase / Acyl-carrier-protein acetyltransferase // [Acyl-carrier-protein] S-malonyltransferase / MCAT // Beta-ketoacyl-[acyl-carrier-protein] synthase I / KAS I // Fatty-acid synthase // Oleoyl-[acyl-carrier-protein] hydrolase / S-acyl fatty acid synthase thioesterase // 3-hydroxyacyl-[acyl-carrier-protein] dehydratase / D-3-hydroxyoctanoyl-[acyl carrier protein] dehydratase; Type I polyketide synthase | CDS | 95 |
| LMJ.RY0402.038466_3 | Cre10.g449750 | PKS1 | (1 of 1) 1.1.1.100//1.3.1.39//2.3.1.38//2.3.1.39//2.3.1.41//2.3.1.85//3.1.2.14//4.2.1.59 - 3-oxoacyl-[acyl-carrier-protein] reductase // Enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) / Enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) // [Acyl-carrier-protein] S-acetyltransferase / Acyl-carrier-protein acetyltransferase // [Acyl-carrier-protein] S-malonyltransferase / MCAT // Beta-ketoacyl-[acyl-carrier-protein] synthase I / KAS I // Fatty-acid synthase // Oleoyl-[acyl-carrier-protein] hydrolase / S-acyl fatty acid synthase thioesterase // 3-hydroxyacyl-[acyl-carrier-protein] dehydratase / D-3-hydroxyoctanoyl-[acyl carrier protein] dehydratase; Type I polyketide synthase | CDS | 95 |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| LAT034H07, screen 1 of 1 (~20.0% growth defect) |
| LAT042F07, screen 1 of 1 (~20.0% growth defect) |
| Paromomycin, screen 3 of 6 (~30.0% growth defect) |
| Paromomycin, screen 4 of 6 (~20.0% growth defect) |
| Paromomycin, screen 5 of 6 (~20.0% growth defect) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.