Mutant strain: LMJ.RY0402.165919

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.165919_1 Cre01.g003550 (1 of 2) PTHR12231//PTHR12231:SF112 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 14, CHLOROPLASTIC intron 73
LMJ.RY0402.165919_2 Cre03.g194200 PDH2 Pyruvate dehydrogenase E1 beta subunit; (1 of 1) PTHR11624//PTHR11624:SF76 - DEHYDROGENASE RELATED // PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT BETA-2, CHLOROPLASTIC-RELATED CDS 73
LMJ.RY0402.165919_3 Cre12.g559350 SDR23 Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.101 - Acylglycerone-phosphate reductase / Palmitoyldihydroxyacetone-phosphate reductase CDS 73

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Carbon concentrating mechanism, screen 8 of 11 (~20.0% growth defect)
Carbon concentrating mechanism, screen 9 of 11 (~20.0% growth defect)
Carbon concentrating mechanism, screen 10 of 11 (No phenotype detected)
Carbon concentrating mechanism, screen 11 of 11 (~10.0% growth defect)
Photosynthesis, screen 1 of 5 (No phenotype detected)
Photosynthesis, screen 3 of 5 (~20.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.