Gene: Cre12.g510100
Overview
| Common name: | ATG4,APG4 |
| Defline: | Autophagy-related protein; (1 of 1) K08342 - cysteine protease ATG4 (ATG4) |
| Description: | Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome generation |
| Synonyms: | APG4,g12348.t1 |
| Chromosome: | chromosome 12 |
Protein Localization
A plasmid for expressing Cre12.g510100 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g510100-Venus-3xFLAG
Imaged strain: CSI_LW12A1
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
cytosol/cytosol (strong signal)
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.028013_1 | CLIP2.028013 | intron | 80 | 6.1 |
| CLIP2.028013_2 | CLIP2.028013 | intron | 80 | 6.1 |
| CLIP2.060893_3 | CLIP2.060893 | 3'UTR | 80 | 6.1 |
| CLIP2.060893_4 | CLIP2.060893 | 3'UTR | 80 | 6.1 |
| CLIP2.063891_1 | CLIP2.063891 | 3'UTR | 80 | 6.1 |
| CLIP2.063891_2 | CLIP2.063891 | 3'UTR | 80 | 6.1 |
| LMJ.RY0402.040385_1 | LMJ.RY0402.040385 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.040385_2 | LMJ.RY0402.040385 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.132136_1 | LMJ.RY0402.132136 | 3'UTR | 58 | 5.5 |
| LMJ.RY0402.132136_2 | LMJ.RY0402.132136 | 3'UTR | 73 | 5.5 |
| LMJ.RY0402.189715_1 | LMJ.RY0402.189715 | CDS | 73 | 5.5 |
| LMJ.RY0402.189715_2 | LMJ.RY0402.189715 | 3'UTR | 73 | 5.5 |
Gene Annotations
| PFAM: | PF03416 |
| PANTHER: | PTHR22624 |
| KEGG_ec: | 3.4.22.- |
| KEGG_orthology: | K08342 |
| KOG: | KOG2674 |
| Gene ontology terms:: | |
| Best arabidopsis TAIR name: | AT3G59950 |
| Best arabidopsis TAIR symbol: | |
| Best arabidopsis TAIR defline: | Peptidase family C54 protein |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.